Oryzanol Testing in Mustard Oil Using HPLC Method

Method validation on HPLC
Oryzanol Testing in Mustard Oil Using HPLC Method

The FSSAI has recently carried out a surveillance exercise of edible oils and fats in Delhi-NCR. According to the sample test reports around 33% samples of mustard oil were found to have the presence of oryzanol, which indicates the presence of rice bran oil in mustard oil. The FSSAI has since directed all Food Safety Commissioners of all States and UTs to carry out enforcement activities and to collect samples of mustard oil of various brands in the market and get them tested for the presence of oryzanol.In case any adulteration is detected then action is to be taken against the defaulters as per the Food Safety and Standards Act, Rules and Regulations.

Rice bran oil is a rich source of oryzanol, whereas mustard oil contains only trace amounts of oryzanol. Therefore, the presence of oryzanol in mustard oil means the mustard oil is not pure but has been adulterated with rice bran oil. Oryzanol is also found in wheat bran and some fruits and vegetables. Moreover, physically refined rice bran oil is similar to mustard oil in colour and density. Since rice bran oil is cheaper than mustard oil it is frequently used as an adulterant in mustard oil.

Arbro Food Testing Laboratory has the capability to test oils and blended oils for adulteration or possible accidental contamination. In bulk handling installations, where oils of different types are pumped through common valves and pipes accidental contamination can occur. However, in order to ensure regulatory requirement of standards for oils it is important to carry out testing of samples. We offer high performance liquid chromatography (HPLC) array detection analyses and testing services for oils to ensure that the samples of mustard oil have the regulatory standards and have not been adulterated with rice bran or any other oil.

Gamma Oryzanol, is a group of ferulic acid esters of phytosterols and triterpene alcohols, is a naturally rich source of antioxidants and unsaturated fatty acids. However, their presence in mustard oil at higher concentrations is a sign of adulteration. HPLC is a testing method that has been developed to detect adulteration in oils, as through this method differences in the profiles of the oils become evident. Also, through HPLC method of testing, concentration of the oryzanol can be detected and so this method is often used to detect rice bran adulteration in mustard oil samples.

Pleases feel free to contact us if you wish to test your Oil samples for adulterants testing.

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